From these results we conclude that 671 bp of the promoter sequence from the ultra-high-sulfur keratin gene is sufficient to direct the correct development-specific and tissue-specific expression of the reporter gene construct in transgenic mice. Improvement of wool production through genetic engineering. Spermatogonia stem cells and surface area in culture with 100ng/mg IGF-1 at days 4, 7, 10 were greater (P <0.05) compared with other treatments or controls. In sheep transgenic for a sheep insulin‐like growth factor 1 (IGF‐1) cDNA driven by a mouse keratin promoter, we assessed wool production and properties in 51 adults of the first generation (G1) and in 56 lambs of the second generation (G2). Register to receive personalised research and resources by email, Wool production in transgenic sheep: Results from first‐generation adults and second‐generation lambs, Animal and Veterinary Sciences Group , Lincoln University , PO Box 84, Canterbury, New Zealand, /doi/pdf/10.1080/10495399809525900?needAccess=true. At second‐and third‐year shearing, however, fleece weights were not significantly different. Many of the growth-promoting properties of GH are mediated by insulin-like growth factor I (IGF-I), a highly conserved circulating 70-amino acid peptide. 2020 Jan 1;98(1):skz381. Transgenic sheep were produced to investigate the possibility that high growth hormone secretion would enhance growth characteristics and aid meat production. 3099067 Darwish HR, El-Shorbagy HM, Abou-Eisha AM, El-Din AE, Farag IM. The promoter was activated by providing zinc to the sheep via drinking water. Expression of CAT activity in skin was correlated with diet-induced or seasonal changes in the rate of wool growth. Two transgenic rams were mated to nontransgenic ewes, and both transmitted the gene to their offspring in Mendelian fashion. Trends Biotechnol. doi: 10.1093/jas/skz381. The experimental information obtained so far in the area indicates that transcriptional regulation of the genes and a thorough understanding of the physiological processes involved are both important factors in the practical application of the technique to the improvement of animal productivity. Transgenic sheep were produced by pronuclear microinjection with a mouse ultra-high-sulfur keratin promoter linked to an ovine insulin-like growth factor 1 (IGF1) cDNA. 2018;14(11):1850-1869. doi: 10.1080/15548627.2018.1490852. Transgenic sheep have been produced expressing a transgene for ovine GH by microinjection of a gene construct possessing the coding region linked to a metal-activated (metallothionein) promoter for controlling expression.
Get the latest research from NIH: https://www.nih.gov/coronavirus. 2017 Dec;15(2):437-441. doi: 10.1016/j.jgeb.2017.08.001. Physiol Rev, 70, 591-614, Hair-specific expression of chloramphenicol aceyltransferase in transgenic mice under the control of an ultra-high-sulfur keratin promoter, The genetic engineering of production traits in domestic animals. One of the nine had high concentrations of immunoassayable hGRF in its plasma and high variable plasma concentrations of ovine growth hormone.
Growth of the single transgenic lamb expressing hGRF was similar to control lambs. This keratin promoter appears useful for overexpressing factors in the wool follicle that might influence wool production or properties. A progeny test of the ram was carried out by matings to 43 non-transgenic ewes. Polymorphism in the ovine keratin-associated protein gene KRTAP7-1 and its association with wool characteristics. The first, MTSGH5, consisted of the sheep metallothionein-Ia promoter joined via a Sall restriction enzyme site to the coding sequence of the …
1996 Feb;14(2):181-4. doi: 10.1038/nbt0296-181.
Pronuclei of Merino sheep embryos were injected with the fusion gene construct ovine metallothionein la-ovine GH. These experiments show that GH enhances IGF-I expression in vivo by predominantly transcriptional mechanisms. New polymorphism in the 5' flanking region of. We use cookies to improve your website experience. This site needs JavaScript to work properly. Registered in England & Wales No. In situ hybridization demonstrated that transgene expression in the skin was confined to the keratogenous zone of the wool follicle cortex. Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era. The wool follicle density and fleece weight were increased. 1994;6(5):615-23. doi: 10.1071/rd9940615.
eCollection 2019. Five transgenic lambs resulted from the microinjection of 591 embryos; one male and one female showed IGF1 expression in the skin. From a total of 435 sheep ova injected with mMThGRF and transferred to recipients, 54 lambs were born and 9 fetuses were collected. J Anim Sci.
Biotechnology (N Y).
A 671-base pair (bp) stretch of the 5' promoter region was used to direct the expression of the CAT gene in this construct. Circulating IGF-1 concentrations were not significantly different between transgenic and nontransgenic animals, suggesting that local mechanisms were more important than systemic mechanisms for wool production, but were significantly higher in males than in females. HHS
The rapid kinetics of IGF-I gene activation and the temporally associated chromatin changes demonstrate a direct link between a GH-dependent signal transduction pathway and nuclear events.
To our knowledge this is the first reported improvement in a production trait by genetic engineering of a farm animal without adverse effects on health or reproduction. transferred. These include the manipulation of the endocrine system by altered growth hormone genes and the modification of animal biochemistry by the introduction of the cysteine biosynthetic pathway and the glyoxylate cycle. A rise in hepatic IGF-I mRNA was detected within 2 h of GH treatment, with peak values of more than 15-fold above untreated animals by 4 h, and a decline by 16 h. A coordinate increase was seen in all IGF-I mRNA splicing and polyadenylation variants, indicating that neither alternative RNA processing nor differential poly A addition were altered by GH. In one lamb, plasma growth hormone was as high as 700 ng/ml. Of 85 lambs born, 43 … Targeting gene expression to the wool follicle in transgenic sheep. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. A progeny test of the ram was carried out by matings to 43 non-transgenic ewes. 2014 Aug;387(8):789-93. doi: 10.1007/s00210-014-0986-0. There were no significant differences in fiber diameter, medullation, and hogget body weight. At second- and third-year shearing, however, fleece weights were not significantly different. Front Genet. Type A spermatogonia were identified by immuno-histochemical staining against PGP9.5 antigen. Nine of the 63 had integrated the mMThGRF gene.
Grading of Wool. increase of 17% in December, but during the winter nadir rates were similar. NLM Epub 2018 Aug 4. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Although the transgene was inherited in Mendelian fashion and was widely expressed, the production advantage seen in animals of the first generation did not persist in the second generation. Please enable it to take advantage of the complete set of features! The transgene was expressed not only in skin but also in a wide range of other tissues. Wool production in transgenic sheep: Res .... Medicine, Dentistry, Nursing & Allied Health. ministry of textiles 106 Table 8.1 Overview of Woollen Industry Total no. Naunyn Schmiedebergs Arch Pharmacol. The results of high-throughput RNA-seq showed that β … Some of the transgenic sheep grew faster and produced more wool than … Transgenic Sheep Produced for the CRC for Premium Quality Wool undergraduate program by; Dr. Kevin Ward, CSIRO Animal Production. Wool production in transgenic sheep: results from first-generation adults and second-generation lambs. |
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